α-synuclein protects naive but not dbcAMP-treated dopaminergic cell types from 1-methyl-4-phenylpyridinium toxicity

Penny J. Jensen, Benedict J. Alter, Karen L. O'Malley

Research output: Contribution to journalArticlepeer-review

37 Scopus citations

Abstract

The pre-synaptic protein, α-synuclein, has been associated with the pathogenesis of Parkinson's disease. The present study indicates that α-synuclein, but not its mutants (A53T, A30P), can protect CNS dopaminergic cells from the parkinsonism-inducing drug 1-methyl-4-phenylpyridinium (MPP+), whereas it cannot protect from the dopaminergic toxin, 6-hydroxydopamine, hydrogen-peroxide, or the β-amyloid peptide, A-β. Protection from MPP+ was directly correlated with the preservation of mitochondrial function. Specifically, α-synuclein rescued cells from MPP+ mediated decreases in mitochondrial dehydrogenase activity and loss of ATP levels by utilizing ketosis. It also prevented toxin-induced activation of the creatine kinase/creatine phosphate system. Similarly, α-synuclein protected cells from the complex I inhibitor rotenone and 3-nitroproprionic acid, a complex II inhibitor. Wild-type α-synuclein-mediated neuroprotection and subsequent alterations in energy were not found in dbcAMP-differentiated cells. These results suggest that the normal physiological role for α-synuclein may change during development.

Original languageEnglish
Pages (from-to)196-209
Number of pages14
JournalJournal of Neurochemistry
Volume86
Issue number1
DOIs
StatePublished - Jul 2003

Keywords

  • Cell death
  • Differentiation
  • MPP
  • Parkinson's disease
  • α-synuclein

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