Research output per year
Research output per year
- 1 - 50 out of 187 results
Search results
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2025
Single-fluorogen imaging reveals distinct environmental and structural features of biomolecular condensates
Wu, T., King, M. R., Qiu, Y., Farag, M., Pappu, R. V. & Lew, M. D., 2025, (Accepted/In press) In: Nature Physics. 5995.Research output: Contribution to journal › Article › peer-review
Open Access -
Synapsin Condensation is Governed by Sequence-Encoded Molecular Grammars
Hoffmann, C., Ruff, K. M., Edu, I. A., Shinn, M. K., Tromm, J. V., King, M. R., Pant, A., Ausserwöger, H., Morgan, J. R., Knowles, T. P. J., Pappu, R. V. & Milovanovic, D., Apr 15 2025, In: Journal of Molecular Biology. 437, 8, 168987.Research output: Contribution to journal › Article › peer-review
Open Access -
2024
6D single-fluorogen orientation-localization microscopy for elucidating the architecture of beta-sheet assemblies and biomolecular condensates
Wu, T., Zhou, W., Rudra, J. S., Pappu, R. V. & Lew, M. D., 2024, High-Speed Biomedical Imaging and Spectroscopy IX. Tsia, K. K. & Goda, K. (eds.). SPIE, 1285302. (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; vol. 12853).Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review
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A High-Avidity, Thermostable, and Low-Cost Synthetic Capture for Ultrasensitive Detection and Quantification of Viral Antigens and Aerosols
Wang, S. J., Gupta, R., Benegal, A., Avula, R., Huang, Y. Y., Vahey, M. D., Chakrabarty, R. K., Pappu, R. V., Singamaneni, S., Puthussery, J. V. & King, M. R., Jun 28 2024, In: ACS Sensors. 9, 6, p. 3096-3104 9 p.Research output: Contribution to journal › Article › peer-review
2 Scopus citations -
Biomolecular Condensates are Characterized by Interphase Electric Potentials
Posey, A. E., Bremer, A., Erkamp, N. A., Pant, A., Knowles, T. P. J., Dai, Y., Mittag, T. & Pappu, R. V., Oct 16 2024, In: Journal of the American Chemical Society. 146, 41, p. 28268-28281 14 p.Research output: Contribution to journal › Article › peer-review
Open Access7 Scopus citations -
Biomolecular condensates form spatially inhomogeneous network fluids
Dar, F., Cohen, S. R., Mitrea, D. M., Phillips, A. H., Nagy, G., Leite, W. C., Stanley, C. B., Choi, J. M., Kriwacki, R. W. & Pappu, R. V., Dec 2024, In: Nature communications. 15, 1, 3413.Research output: Contribution to journal › Article › peer-review
Open Access8 Scopus citations -
Crowder titrations enable the quantification of driving forces for macromolecular phase separation
Chauhan, G., Bremer, A., Dar, F., Mittag, T. & Pappu, R. V., Jun 4 2024, In: Biophysical Journal. 123, 11, p. 1376-1392 17 p.Research output: Contribution to journal › Article › peer-review
Open Access7 Scopus citations -
Direct computations of viscoelastic moduli of biomolecular condensates
Cohen, S. R., Banerjee, P. R. & Pappu, R. V., Sep 7 2024, In: Journal of Chemical Physics. 161, 9, 095103.Research output: Contribution to journal › Article › peer-review
Open Access3 Scopus citations -
Dominance analysis to assess solute contributions to multicomponent phase equilibria
Qian, D., Ausserwoger, H., Sneideris, T., Farag, M., Pappu, R. V. & Knowles, T. P. J., Aug 13 2024, In: Proceedings of the National Academy of Sciences of the United States of America. 121, 33, e2407453121.Research output: Contribution to journal › Article › peer-review
Open Access1 Scopus citations -
Emergent microenvironments of nucleoli
King, M. R., Ruff, K. M. & Pappu, R. V., 2024, In: Nucleus (United States). 15, 1, 2319957.Research output: Contribution to journal › Review article › peer-review
Open Access13 Scopus citations -
Identifying Sequence Effects on Chain Dimensions of Disordered Proteins by Integrating Experiments and Simulations
Holla, A., Martin, E. W., Dannenhoffer-Lafage, T., Ruff, K. M., König, S. L. B., Nüesch, M. F., Chowdhury, A., Louis, J. M., Soranno, A., Nettels, D., Pappu, R. V., Best, R. B., Mittag, T. & Schuler, B., Dec 23 2024, In: JACS Au. 4, 12, p. 4729-4743 15 p.Research output: Contribution to journal › Article › peer-review
Open Access -
Intrinsic disorder: A term to define the specific physicochemical characteristic of protein conformational heterogeneity
Lemke, E. A., Babu, M. M., Kriwacki, R. W., Mittag, T., Pappu, R. V., Wright, P. E. & Forman-Kay, J. D., Apr 4 2024, In: Molecular cell. 84, 7, p. 1188-1190 3 p.Research output: Contribution to journal › Comment/debate
Open Access4 Scopus citations -
Macromolecular condensation organizes nucleolar sub-phases to set up a pH gradient
King, M. R., Ruff, K. M., Lin, A. Z., Pant, A., Farag, M., Lalmansingh, J. M., Wu, T., Fossat, M. J., Ouyang, W., Lew, M. D., Lundberg, E., Vahey, M. D. & Pappu, R. V., Apr 11 2024, In: Cell. 187, 8, p. 1889-1906.e24Research output: Contribution to journal › Article › peer-review
Open Access38 Scopus citations -
Reply to: The conformations of protein chains at the interface of biomolecular condensates
Chauhan, G., Farag, M., Cohen, S. R. & Pappu, R. V., Dec 2024, In: Nature communications. 15, 1, 9974.Research output: Contribution to journal › Letter › peer-review
Open Access -
Sequence-specific interactions determine viscoelasticity and ageing dynamics of protein condensates
Alshareedah, I., Borcherds, W. M., Cohen, S. R., Singh, A., Posey, A. E., Farag, M., Bremer, A., Strout, G. W., Tomares, D. T., Pappu, R. V., Mittag, T. & Banerjee, P. R., Sep 2024, In: Nature Physics. 20, 9, p. 1482-1491 10 p.Research output: Contribution to journal › Article › peer-review
Open Access22 Scopus citations -
Solutes unmask differences in clustering versus phase separation of FET proteins
Kar, M., Vogel, L. T., Chauhan, G., Felekyan, S., Ausserwöger, H., Welsh, T. J., Dar, F., Kamath, A. R., Knowles, T. P. J., Hyman, A. A., Seidel, C. A. M. & Pappu, R. V., Dec 2024, In: Nature communications. 15, 1, 4408.Research output: Contribution to journal › Article › peer-review
Open Access6 Scopus citations -
Tear down this wall: phosphorylation regulates the internal interfaces of postsynaptic condensates
Aguilar Pérez, G., Pappu, R. V. & Milovanovic, D., Apr 2024, In: Trends in Cell Biology. 34, 4, p. 274-276 3 p.Research output: Contribution to journal › Short survey › peer-review
Open Access -
2023
A disordered region controls cBAF activity via condensation and partner recruitment
Patil, A., Strom, A. R., Paulo, J. A., Collings, C. K., Ruff, K. M., Shinn, M. K., Sankar, A., Cervantes, K. S., Wauer, T., St. Laurent, J. D., Xu, G., Becker, L. A., Gygi, S. P., Pappu, R. V., Brangwynne, C. P. & Kadoch, C., Oct 26 2023, In: Cell. 186, 22, p. 4936-4955.e26Research output: Contribution to journal › Article › peer-review
Open Access57 Scopus citations -
Developments in describing equilibrium phase transitions of multivalent associative macromolecules
Zeng, X. & Pappu, R. V., Apr 2023, In: Current Opinion in Structural Biology. 79, 102540.Research output: Contribution to journal › Review article › peer-review
Open Access14 Scopus citations -
Dynamical control enables the formation of demixed biomolecular condensates
Lin, A. Z., Ruff, K. M., Dar, F., Jalihal, A., King, M. R., Lalmansingh, J. M., Posey, A. E., Erkamp, N. A., Seim, I., Gladfelter, A. S. & Pappu, R. V., Dec 2023, In: Nature communications. 14, 1, 7678.Research output: Contribution to journal › Article › peer-review
Open Access21 Scopus citations -
FIREBALL: A tool to fit protein phase diagrams based on mean-field theories for polymer solutions
Farag, M., Holehouse, A. S., Zeng, X. & Pappu, R. V., Jun 20 2023, In: Biophysical Journal. 122, 12, p. 2396-2403 8 p.Research output: Contribution to journal › Article › peer-review
Open Access6 Scopus citations -
Phase separation of protein mixtures is driven by the interplay of homotypic and heterotypic interactions
Farag, M., Borcherds, W. M., Bremer, A., Mittag, T. & Pappu, R. V., Dec 2023, In: Nature communications. 14, 1, 5527.Research output: Contribution to journal › Article › peer-review
Open Access50 Scopus citations -
Phase Transitions of Associative Biomacromolecules
Pappu, R. V., Cohen, S. R., Dar, F., Farag, M. & Kar, M., Jul 26 2023, In: Chemical Reviews. 123, 14, p. 8945-8987 43 p.Research output: Contribution to journal › Review article › peer-review
Open Access135 Scopus citations -
Programmable synthetic biomolecular condensates for cellular control
Dai, Y., Farag, M., Lee, D., Zeng, X., Kim, K., Son, H. I., Guo, X., Su, J., Peterson, N., Mohammed, J., Ney, M., Shapiro, D. M., Pappu, R. V., Chilkoti, A. & You, L., Apr 2023, In: Nature Chemical Biology. 19, 4, p. 518-528 11 p.Research output: Contribution to journal › Article › peer-review
Open Access64 Scopus citations -
RNAs undergo phase transitions with lower critical solution temperatures
Wadsworth, G. M., Zahurancik, W. J., Zeng, X., Pullara, P., Lai, L. B., Sidharthan, V., Pappu, R. V., Gopalan, V. & Banerjee, P. R., Dec 2023, In: Nature Chemistry. 15, 12, p. 1693-1704 12 p.Research output: Contribution to journal › Article › peer-review
Open Access38 Scopus citations -
Soaping up transcriptional condensates
Shinn, M. K. & Pappu, R. V., Jun 5 2023, In: Developmental cell. 58, 11, p. 915-916 2 p.Research output: Contribution to journal › Comment/debate
Open Access2 Scopus citations -
SOURSOP: A Python Package for the Analysis of Simulations of Intrinsically Disordered Proteins
Lalmansingh, J. M., Keeley, A. T., Ruff, K. M., Pappu, R. V. & Holehouse, A. S., Aug 22 2023, In: Journal of Chemical Theory and Computation. 19, 16, p. 5609-5620 12 p.Research output: Contribution to journal › Article › peer-review
Open Access13 Scopus citations -
Uncovering the Contributions of Charge Regulation to the Stability of Single Alpha Helices**
Fossat, M. J., Posey, A. E. & Pappu, R. V., Apr 3 2023, In: ChemPhysChem. 24, 7, e202200746.Research output: Contribution to journal › Article › peer-review
Open Access10 Scopus citations -
2022
A conceptual framework for understanding phase separation and addressing open questions and challenges
Mittag, T. & Pappu, R. V., Jun 16 2022, In: Molecular cell. 82, 12, p. 2201-2214 14 p.Research output: Contribution to journal › Review article › peer-review
Open Access285 Scopus citations -
Competing interactions give rise to two-state behavior and switch-like transitions in charge-rich intrinsically disordered proteins
Zeng, X., Ruff, K. M. & Pappu, R. V., May 10 2022, In: Proceedings of the National Academy of Sciences of the United States of America. 119, 19, e2200559119.Research output: Contribution to journal › Article › peer-review
Open Access38 Scopus citations -
Condensates formed by prion-like low-complexity domains have small-world network structures and interfaces defined by expanded conformations
Farag, M., Cohen, S. R., Borcherds, W. M., Bremer, A., Mittag, T. & Pappu, R. V., Dec 2022, In: Nature communications. 13, 1, 7722.Research output: Contribution to journal › Article › peer-review
Open Access88 Scopus citations -
Conformational buffering underlies functional selection in intrinsically disordered protein regions
González-Foutel, N. S., Glavina, J., Borcherds, W. M., Safranchik, M., Barrera-Vilarmau, S., Sagar, A., Estaña, A., Barozet, A., Garrone, N. A., Fernandez-Ballester, G., Blanes-Mira, C., Sánchez, I. E., de Prat-Gay, G., Cortés, J., Bernadó, P., Pappu, R. V., Holehouse, A. S., Daughdrill, G. W. & Chemes, L. B., Aug 2022, In: Nature Structural and Molecular Biology. 29, 8, p. 781-790 10 p.Research output: Contribution to journal › Article › peer-review
Open Access53 Scopus citations -
Connecting sequence features within the disordered C-terminal linker of Bacillus subtilis FtsZ to functions and bacterial cell division
Shinn, M. K., Cohan, M. C., Bullock, J. L., Ruff, K. M., Levin, P. A. & Pappu, R. V., Oct 18 2022, In: Proceedings of the National Academy of Sciences of the United States of America. 119, 42, e2211178119.Research output: Contribution to journal › Article › peer-review
Open Access12 Scopus citations -
Deciphering how naturally occurring sequence features impact the phase behaviours of disordered prion-like domains
Bremer, A., Farag, M., Borcherds, W. M., Peran, I., Martin, E. W., Pappu, R. V. & Mittag, T., Feb 2022, In: Nature Chemistry. 14, 2, p. 196-207 12 p.Research output: Contribution to journal › Article › peer-review
Open Access220 Scopus citations -
Dilute phase oligomerization can oppose phase separation and modulate material properties of a ribonucleoprotein condensate
Seim, I., Posey, A. E., Snead, W. T., Stormo, B. M., Klotsa, D., Pappu, R. V. & Gladfelter, A. S., Mar 29 2022, In: Proceedings of the National Academy of Sciences of the United States of America. 119, 13, e2120799119.Research output: Contribution to journal › Article › peer-review
Open Access47 Scopus citations -
Directed mutational scanning reveals a balance between acidic and hydrophobic residues in strong human activation domains
Staller, M., Ramirez, E., Kotha, S. R., Holehouse, A. S., Pappu, R. V. & Cohen, B. A., Apr 20 2022, In: Cell Systems. 13, 4, p. 334-345.e5Research output: Contribution to journal › Article › peer-review
Open Access56 Scopus citations -
Mesoscale structure–function relationships in mitochondrial transcriptional condensates
Feric, M., Sarfallah, A., Dar, F., Temiakov, D., Pappu, R. V. & Misteli, T., Oct 11 2022, In: Proceedings of the National Academy of Sciences of the United States of America. 119, 41, e2207303119.Research output: Contribution to journal › Article › peer-review
Open Access22 Scopus citations -
Phase-separating RNA-binding proteins form heterogeneous distributions of clusters in subsaturated solutions
Kar, M., Dar, F., Welsh, T. J., Vogel, L. T., Kühnemuth, R., Majumdar, A., Krainer, G., Franzmann, T. M., Alberti, S., Seidel, C. A. M., Knowles, T. P. J., Hyman, A. A. & Pappu, R. V., Jul 12 2022, In: Proceedings of the National Academy of Sciences of the United States of America. 119, 28, e2202222119.Research output: Contribution to journal › Article › peer-review
Open Access121 Scopus citations -
Quantifying Coexistence Concentrations in Multi-Component Phase-Separating Systems Using Analytical HPLC
Bremer, A., Posey, A. E., Borgia, M. B., Borcherds, W. M., Farag, M., Pappu, R. V. & Mittag, T., Oct 2022, In: Biomolecules. 12, 10, 1480.Research output: Contribution to journal › Article › peer-review
Open Access10 Scopus citations -
Sequence grammar underlying the unfolding and phase separation of globular proteins
Ruff, K. M., Choi, Y. H., Cox, D., Ormsby, A. R., Myung, Y., Ascher, D. B., Radford, S. E., Pappu, R. V. & Hatters, D. M., Sep 1 2022, In: Molecular cell. 82, 17, p. 3193-3208.e8Research output: Contribution to journal › Article › peer-review
Open Access51 Scopus citations -
Uncovering Non-random Binary Patterns Within Sequences of Intrinsically Disordered Proteins
Cohan, M. C., Shinn, M. K., Lalmansingh, J. M. & Pappu, R. V., Jan 30 2022, In: Journal of Molecular Biology. 434, 2, 167373.Research output: Contribution to journal › Article › peer-review
Open Access48 Scopus citations -
2021
AlphaFold and Implications for Intrinsically Disordered Proteins
Ruff, K. M. & Pappu, R. V., Oct 1 2021, In: Journal of Molecular Biology. 433, 20, 167208.Research output: Contribution to journal › Review article › peer-review
Open Access342 Scopus citations -
Design of intrinsically disordered proteins that undergo phase transitions with lower critical solution temperatures
Zeng, X., Liu, C., Fossat, M. J., Ren, P., Chilkoti, A. & Pappu, R. V., Feb 1 2021, In: APL Materials. 9, 2, 021119.Research output: Contribution to journal › Article › peer-review
Open Access26 Scopus citations -
Endocytic proteins with prion-like domains form viscoelastic condensates that enable membrane remodeling
Bergeron-Sandoval, L. P., Kumar, S., Heris, H. K., Chang, C. L. A., Cornell, C. E., Keller, S. L., François, P., Hendricks, A. G., Ehrlicher, A. J., Pappu, R. V. & Michnick, S. W., Dec 14 2021, In: Proceedings of the National Academy of Sciences of the United States of America. 118, 50, e2113789118.Research output: Contribution to journal › Article › peer-review
Open Access88 Scopus citations -
Faces, facets, and functions of biomolecular condensates driven by multivalent proteins and nucleic acids
Kahn, J. D., Lemke, E. A. & Pappu, R. V., Apr 6 2021, In: Biophysical Journal. 120, 7, p. E1-E4Research output: Contribution to journal › Editorial
Open Access1 Scopus citations -
Glycine-Rich Peptides from FUS Have an Intrinsic Ability to Self-Assemble into Fibers and Networked Fibrils
Kar, M., Posey, A. E., Dar, F., Hyman, A. A. & Pappu, R. V., Nov 2 2021, In: Biochemistry. 60, 43, p. 3213-3222 10 p.Research output: Contribution to journal › Article › peer-review
Open Access26 Scopus citations -
Ligand effects on phase separation of multivalent macromolecules
Ruff, K. M., Dar, F. & Pappu, R. V., Mar 9 2021, In: Proceedings of the National Academy of Sciences of the United States of America. 118, 10, e2017184118.Research output: Contribution to journal › Article › peer-review
Open Access84 Scopus citations -
Liquid-Liquid Phase Separation: A Widespread and Versatile Way to Organize Aqueous Solutions
Keating, C. D. & Pappu, R. V., Nov 18 2021, In: Journal of Physical Chemistry Letters. 12, 45, p. 10994-10995 2 p.Research output: Contribution to journal › Editorial
8 Scopus citations -
Liquid-Liquid Phase Separation: A Widespread and Versatile Way to Organize Aqueous Solutions
Keating, C. D. & Pappu, R. V., Nov 18 2021, In: Journal of Physical Chemistry B. 125, 45, p. 12399-12400 2 p.Research output: Contribution to journal › Editorial
Open Access7 Scopus citations -
Mechanistic Inferences From Analysis of Measurements of Protein Phase Transitions in Live Cells
Posey, A. E., Ruff, K. M., Lalmansingh, J. M., Kandola, T. S., Lange, J. J., Halfmann, R. & Pappu, R. V., Jun 11 2021, In: Journal of Molecular Biology. 433, 12, 166848.Research output: Contribution to journal › Article › peer-review
Open Access14 Scopus citations