Haojia Wu

2025

Histopathologic Analysis of Human Kidney Spatial Transcriptomics Data: Toward Precision Pathology

Isnard, P., Li, D., Xuanyuan, Q., Wu, H. & Humphreys, B. D., Jan 2025, In: American Journal of Pathology. 195, 1, p. 69-88 20 p.

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

2024

A genetically inducible endothelial niche enables vascularization of human kidney organoids with multilineage maturation and emergence of renin expressing cells

Maggiore, J. C., LeGraw, R., Przepiorski, A., Velazquez, J., Chaney, C., Vanichapol, T., Streeter, E., Almuallim, Z., Oda, A., Chiba, T., Silva-Barbosa, A., Franks, J., Hislop, J., Hill, A., Wu, H., Pfister, K., Howden, S. E., Watkins, S. C., Little, M. H. & Humphreys, B. D. & 9 others, Kiani, S., Watson, A., Stolz, D. B., Davidson, A. J., Carroll, T., Cleaver, O., Sims-Lucas, S., Ebrahimkhani, M. R. & Hukriede, N. A., Dec 2024, In: Kidney International. 106, 6, p. 1086-1100 15 p.

Research output: Contribution to journal › Article › peer-review

Open Access

13 Scopus citations

Emerging high-resolution spatial transcriptomic technologies in kidney research

Xuanyuan, Q., Wu, H. & Humphreys, B. D., Nov 1 2024, In: Nephrology Dialysis Transplantation. 39, 11, p. 1747-1750 4 p.

Research output: Contribution to journal › Editorial

Open Access

Epigenetic reprogramming driving successful and failed repair in acute kidney injury

Muto, Y., Dixon, E. E., Yoshimura, Y., Ledru, N., Kirita, Y., Wu, H. & Humphreys, B. D., Aug 2024, In: Science Advances. 10, 32, ado2849.

Research output: Contribution to journal › Article › peer-review

Open Access

14 Scopus citations

High resolution spatial profiling of kidney injury and repair using RNA hybridization-based in situ sequencing

Wu, H., Dixon, E. E., Xuanyuan, Q., Guo, J., Yoshimura, Y., Debashish, C., Niesnerova, A., Xu, H., Rouault, M. & Humphreys, B. D., Dec 2024, In: Nature communications. 15, 1, 1396.

Research output: Contribution to journal › Article › peer-review

Open Access

18 Scopus citations

Injury-induced Foxm1 expression in the mouse kidney drives epithelial proliferation by a cyclin F-dependent mechanism

Noonan, M. L., Muto, Y., Yoshimura, Y., Leckie-Harr, A., Wu, H., Kalinichenko, V. V., Humphreys, B. D. & Monica, C. P., 2024, In: JCI Insight. 9, 15, e175416.

Research output: Contribution to journal › Article › peer-review

Open Access

Multiomics profiling of mouse polycystic kidney disease progression at a single-cell resolution

Muto, Y., Yoshimura, Y., Wu, H., Chang-Panesso, M., Ledru, N., Woodward, O. M., Outeda, P., Cheng, T., Mahjoub, M., Watnick, T. J. & Humphreys, B., Oct 22 2024, In: Proceedings of the National Academy of Sciences of the United States of America. 121, 43, e2410830121.

Research output: Contribution to journal › Article › peer-review

Open Access

3 Scopus citations

Predicting proximal tubule failed repair drivers through regularized regression analysis of single cell multiomic sequencing

Ledru, N., Wilson, P. C., Muto, Y., Yoshimura, Y., Wu, H., Li, D., Asthana, A., Tullius, S. G., Waikar, S. S., Orlando, G. & Humphreys, B. D., Dec 2024, In: Nature communications. 15, 1, 1291.

Research output: Contribution to journal › Article › peer-review

Open Access

24 Scopus citations

Sequencing of Physically Interacting Cells in Human Kidney Allograft Rejection to Infer Contact-dependent Immune Cell Transcription

Leckie-Harre, A., Silverman, I., Wu, H., Humphreys, B. D. & Malone, A. F., Feb 1 2024, In: Transplantation. 108, 2, p. 421-429 9 p.

Research output: Contribution to journal › Article › peer-review

Open Access

9 Scopus citations

TNIK depletion induces inflammation and apoptosis in injured renal proximal tubule epithelial cells

Bradford, S. T. J., Wu, H., Kirita, Y., Chen, C., Malvin, N. P., Yoshimura, Y., Muto, Y. & Humphreys, B. D., May 2024, In: American Journal of Physiology - Renal Fluid and Electrolyte Physiology. 326, 5, p. F827-F838

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Transcriptomic, epigenomic, and spatial metabolomic cell profiling redefines regional human kidney anatomy

Li, H., Li, D., Ledru, N., Xuanyuan, Q., Wu, H., Asthana, A., Byers, L. N., Tullius, S. G., Orlando, G., Waikar, S. S. & Humphreys, B. D., May 7 2024, In: Cell metabolism. 36, 5, p. 1105-1125.e10

Research output: Contribution to journal › Article › peer-review

Open Access

29 Scopus citations

2023

A single-cell multiomic analysis of kidney organoid differentiation

Yoshimura, Y., Muto, Y., Ledru, N., Wu, H., Omachi, K., Miner, J. H. & Humphreys, B. D., May 16 2023, In: Proceedings of the National Academy of Sciences of the United States of America. 120, 20, e2219699120.

Research output: Contribution to journal › Article › peer-review

Open Access

21 Scopus citations

Lineage Tracing and Single-Nucleus Multiomics Reveal Novel Features of Adaptive and Maladaptive Repair after Acute Kidney Injury

Gerhardt, L. M. S., Koppitch, K., Van Gestel, J., Guo, J., Cho, S., Wu, H., Kirita, Y., Humphreys, B. D. & McMahon, A. P., Apr 1 2023, In: Journal of the American Society of Nephrology. 34, 4, p. 554-571 18 p.

Research output: Contribution to journal › Article › peer-review

Open Access

53 Scopus citations

2022

Comprehensive single-cell transcriptional profiling defines shared and unique epithelial injury responses during kidney fibrosis

Li, H., Dixon, E. E., Wu, H. & Humphreys, B. D., Dec 6 2022, In: Cell metabolism. 34, 12, p. 1977-1998.e9

Research output: Contribution to journal › Article › peer-review

Open Access

116 Scopus citations

Defining cellular complexity in human autosomal dominant polycystic kidney disease by multimodal single cell analysis

Muto, Y., Dixon, E. E., Yoshimura, Y., Wu, H., Omachi, K., Ledru, N., Wilson, P. C., King, A. J., Eric Olson, N., Gunawan, M. G., Kuo, J. J., Cox, J. H., Miner, J. H., Seliger, S. L., Woodward, O. M., Welling, P. A., Watnick, T. J. & Humphreys, B. D., Dec 2022, In: Nature communications. 13, 1, 6497.

Research output: Contribution to journal › Article › peer-review

52 Scopus citations

Immune cell heterogeneity in a mouse model of diabetic kidney disease

Wu, H. & Humphreys, B. D., Dec 2022, In: Kidney International. 102, 6, p. 1215-1216 2 p.

Research output: Contribution to journal › Comment/debate

6 Scopus citations

Mapping the single-cell transcriptomic response of murine diabetic kidney disease to therapies

Wu, H., Villalobos, R. G., Yao, X., Reilly, D., Chen, T., Rankin, M., Myshkin, E., Breyer, M. D. & Humphreys, B. D., Jul 5 2022, In: Cell metabolism. 34, 7, p. 1064-1078.e6

Research output: Contribution to journal › Article › peer-review

Open Access

146 Scopus citations

Multimodal single cell sequencing implicates chromatin accessibility and genetic background in diabetic kidney disease progression

Wilson, P. C., Muto, Y., Wu, H., Karihaloo, A., Waikar, S. S. & Humphreys, B. D., Dec 2022, In: Nature communications. 13, 1, 5253.

Research output: Contribution to journal › Article › peer-review

Open Access

92 Scopus citations

Spatially Resolved Transcriptomic Analysis of Acute Kidney Injury in a Female Murine Model

Dixon, E. E., Wu, H., Muto, Y., Wilson, P. C. & Humphreys, B. D., Feb 2022, In: Journal of the American Society of Nephrology. 33, 2

Research output: Contribution to journal › Article › peer-review

Open Access

83 Scopus citations

Spatially resolved transcriptomics and the kidney: many opportunities

Dixon, E. E., Wu, H., Sulvarán-Guel, E., Guo, J. & Humphreys, B. D., Sep 2022, In: Kidney International. 102, 3, p. 482-491 10 p.

Research output: Contribution to journal › Short survey › peer-review

21 Scopus citations

2021

Cadherin-11, Sparc-related modular calcium binding protein-2, and Pigment epithelium-derived factor are promising non-invasive biomarkers of kidney fibrosis

Kidney Precision Medicine Project (KPMP), Sep 2021, In: Kidney International. 100, 3, p. 672-683 12 p.

Research output: Contribution to journal › Article › peer-review

32 Scopus citations

Single cell transcriptional and chromatin accessibility profiling redefine cellular heterogeneity in the adult human kidney

Muto, Y., Wilson, P. C., Ledru, N., Wu, H., Dimke, H., Waikar, S. S. & Humphreys, B. D., Dec 1 2021, In: Nature communications. 12, 1, 2190.

Research output: Contribution to journal › Article › peer-review

Open Access

289 Scopus citations

2020

Cell profiling of mouse acute kidney injury reveals conserved cellular responses to injury

Kirita, Y., Wu, H., Uchimura, K., Wilson, P. C. & Humphreys, B. D., Jul 7 2020, In: Proceedings of the National Academy of Sciences of the United States of America. 117, 27, p. 15874-15883 10 p.

Research output: Contribution to journal › Article › peer-review

Open Access

376 Scopus citations

Harnessing Expressed Single Nucleotide Variation and Single Cell RNA Sequencing to Define Immune Cell Chimerism in the Rejecting Kidney Transplant

Malone, A. F., Wu, H., Fronick, C., Fulton, R., Gaut, J. P. & Humphreys, B. D., Sep 2020, In: Journal of the American Society of Nephrology. 31, 9, p. 1977-1986 10 p.

Research output: Contribution to journal › Article › peer-review

Open Access

80 Scopus citations

Human Pluripotent Stem Cell-Derived Kidney Organoids with Improved Collecting Duct Maturation and Injury Modeling

Uchimura, K., Wu, H., Yoshimura, Y. & Humphreys, B. D., Dec 15 2020, In: Cell Reports. 33, 11, 108514.

Research output: Contribution to journal › Article › peer-review

Open Access

95 Scopus citations

Proximal tubule translational profiling during kidney fibrosis reveals proinflammatory and long noncoding RNA expression patterns with sexual dimorphism

Wu, H., Lai, C. F., Chang-Panesso, M. & Humphreys, B. D., Jan 2020, In: Journal of the American Society of Nephrology. 31, 1, p. 23-38 16 p.

Research output: Contribution to journal › Article › peer-review

67 Scopus citations

Single cell sequencing and kidney organoids generated from pluripotent stem cells

Wu, H. & Humphreys, B. D., Apr 1 2020, In: Clinical Journal of the American Society of Nephrology. 15, 4, p. 550-556 7 p.

Research output: Contribution to journal › Article › peer-review

Open Access

23 Scopus citations

Single-nucleus RNA-sequencing profiling of mouse lung reduced dissociation bias and improved rare cell-type detection compared with single-cell RNA sequencing

Koenitzer, J. R., Wu, H., Atkinson, J. J., Brody, S. L. & Humphreys, B. D., Dec 2020, In: American Journal of Respiratory Cell and Molecular Biology. 63, 6, p. 739-747 9 p.

Research output: Contribution to journal › Article › peer-review

Open Access

48 Scopus citations

2019

A conditionally immortalized Gli1-positive kidney mesenchymal cell line models myofibroblast transition

Ó Hainmhire, E., Wu, H., Muto, Y., Donnelly, E. L., Machado, F. G., Fan, L. X., Chang-Panesso, M. & Humphreys, B. D., Jan 2019, In: American Journal of Physiology - Renal Fluid and Electrolyte Physiology. 316, 1, p. F63-F75

Research output: Contribution to journal › Article › peer-review

Open Access

18 Scopus citations

Advantages of single-nucleus over single-cell RNA sequencing of adult kidney: Rare cell types and novel cell states revealed in fibrosis

Wu, H., Kirita, Y., Donnelly, E. L. & Humphreys, B. D., Jan 2019, In: Journal of the American Society of Nephrology. 30, 1, p. 23-32 10 p.

Research output: Contribution to journal › Article › peer-review

Open Access

511 Scopus citations

Authors’ reply

Wu, H., Kirita, Y., Donnelly, E. L. & Humphreys, B. D., Apr 2019, In: Journal of the American Society of Nephrology. 30, 4, p. 714 1 p.

Research output: Contribution to journal › Letter › peer-review

Open Access

2 Scopus citations

FOXM1 drives proximal tubule proliferation during repair from acute ischemic kidney injury

Chang-Panesso, M., Kadyrov, F. F., Lalli, M., Wu, H., Ikeda, S., Kefaloyianni, E., Abdelmageed, M. M., Herrlich, A., Kobayashi, A. & Humphreys, B. D., Dec 2 2019, In: Journal of Clinical Investigation. 129, 12, p. 5501-5517 17 p.

Research output: Contribution to journal › Article › peer-review

Open Access

124 Scopus citations

The single-cell transcriptomic landscape of early human diabetic nephropathy

Wilson, P. C., Wu, H., Kirita, Y., Uchimura, K., Ledru, N., Rennke, H. G., Welling, P. A., Waikar, S. S. & Humphreys, B. D., Sep 24 2019, In: Proceedings of the National Academy of Sciences of the United States of America. 116, 39, p. 19619-19625 7 p.

Research output: Contribution to journal › Article › peer-review

Open Access

369 Scopus citations

Trans-ethnic kidney function association study reveals putative causal genes and effects on kidney-specific disease aetiologies

Morris, A. P., Le, T. H., Wu, H., Akbarov, A., van der Most, P. J., Hemani, G., Smith, G. D., Mahajan, A., Gaulton, K. J., Nadkarni, G. N., Valladares-Salgado, A., Wacher-Rodarte, N., Mychaleckyj, J. C., Dueker, N. D., Guo, X., Hai, Y., Haessler, J., Kamatani, Y., Stilp, A. M. & Zhu, G. & 52 others, Cook, J. P., Ärnlöv, J., Blanton, S. H., de Borst, M. H., Bottinger, E. P., Buchanan, T. A., Cechova, S., Charchar, F. J., Chu, P. L., Damman, J., Eales, J., Gharavi, A. G., Giedraitis, V., Heath, A. C., Ipp, E., Kiryluk, K., Kramer, H. J., Kubo, M., Larsson, A., Lindgren, C. M., Lu, Y., Madden, P. A. F., Montgomery, G. W., Papanicolaou, G. J., Raffel, L. J., Sacco, R. L., Sanchez, E., Stark, H., Sundstrom, J., Taylor, K. D., Xiang, A. H., Zivkovic, A., Lind, L., Ingelsson, E., Martin, N. G., Whitfield, J. B., Cai, J., Laurie, C. C., Okada, Y., Matsuda, K., Kooperberg, C., Chen, Y. D. I., Rundek, T., Rich, S. S., Loos, R. J. F., Parra, E. J., Cruz, M., Rotter, J. I., Snieder, H., Tomaszewski, M., Humphreys, B. D. & Franceschini, N., Dec 1 2019, In: Nature communications. 10, 1, 29.

Research output: Contribution to journal › Article › peer-review

105 Scopus citations

2018

Bringing Renal Biopsy Interpretation Into the Molecular Age With Single-Cell RNA Sequencing

Malone, A. F., Wu, H. & Humphreys, B. D., Jan 2018, In: Seminars in Nephrology. 38, 1, p. 31-39 9 p.

Research output: Contribution to journal › Review article › peer-review

Open Access

30 Scopus citations

Comparative Analysis and Refinement of Human PSC-Derived Kidney Organoid Differentiation with Single-Cell Transcriptomics

Wu, H., Uchimura, K., Donnelly, E. L., Kirita, Y., Morris, S. A. & Humphreys, B. D., Dec 6 2018, In: Cell Stem Cell. 23, 6, p. 869-881.e8

Research output: Contribution to journal › Article › peer-review

Open Access

435 Scopus citations

Complement C5a inhibition moderates lipid metabolism and reduces tubulointerstitial fibrosis in diabetic nephropathy

Yiu, W. H., Li, R. X., Wong, D. W. L., Wu, H. J., Chan, K. W., Chan, L. Y. Y., Leung, J. C. K., Lai, K. N., Sacks, S. H., Zhou, W. & Tang, S. C. W., Aug 1 2018, In: Nephrology Dialysis Transplantation. 33, 8, p. 1323-1332 10 p.

Research output: Contribution to journal › Article › peer-review

Open Access

79 Scopus citations

N-acetyl-seryl-aspartyl-lysyl-proline mediates the anti-fibrotic properties of captopril in unilateral ureteric obstructed BALB/C mice

Chan, G. C. W., Wu, H. J., Chan, K. W., Yiu, W. H., Zou, A., Huang, X. R., Lan, H. Y., Lai, K. N. & Tang, S. C. W., Apr 2018, In: Nephrology. 23, 4, p. 297-307 11 p.

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Parabiosis and single-cell RNA sequencing reveal a limited contribution of monocytes to myofibroblasts in kidney fibrosis

Kramann, R., Machado, F., Wu, H., Kusaba, T., Hoeft, K., Schneider, R. K. & Humphreys, B. D., May 3 2018, In: JCI Insight. 3, 9

Research output: Contribution to journal › Article › peer-review

Open Access

76 Scopus citations

Single-cell transcriptomics of a human kidney allograft biopsy specimen defines a diverse inflammatory response

Wu, H., Malone, A. F., Donnelly, E. L., Kirita, Y., Uchimura, K., Ramakrishnan, S. M., Gaut, J. P. & Humphreys, B. D., Aug 2018, In: Journal of the American Society of Nephrology. 29, 8, p. 2069-2080 12 p.

Research output: Contribution to journal › Article › peer-review

303 Scopus citations

2017

Human induced pluripotent stem cell-derived mesenchymal stem cells prevent adriamycin nephropathy in mice

Wu, H. J., Yiu, W. H., Wong, D. W. L., Li, R. X., Chan, L. Y. Y., Leung, J. C. K., Zhang, Y., Lian, Q., Lai, K. N., Tse, H. F. & Tang, S. C. W., 2017, In: Oncotarget. 8, 61, p. 103640-103656 17 p.

Research output: Contribution to journal › Article › peer-review

Open Access

18 Scopus citations

The promise of single-cell RNA sequencing for kidney disease investigation

Wu, H. & Humphreys, B. D., Dec 2017, In: Kidney International. 92, 6, p. 1334-1342 9 p.

Research output: Contribution to journal › Short survey › peer-review

Open Access

68 Scopus citations

2016

Downregulation of renal tubular Wnt/β-catenin signaling by Dickkopf-3 induces tubular cell death in proteinuric nephropathy

Wong, D. W. L., Yiu, W. H., Wu, H. J., Li, R. X., Liu, Y., Chan, K. W., Leung, J. C. K., Chan, L. Y. Y., Lai, K. N. & Tang, S. C. W., 2016, In: Cell Death and Disease. 7, 3, e2155.

Research output: Contribution to journal › Article › peer-review

Open Access

21 Scopus citations

Kallistatin protects against diabetic nephropathy in db/db mice by suppressing AGE-RAGE-induced oxidative stress

Yiu, W. H., Wong, D. W. L., Wu, H. J., Li, R. X., Yam, I., Chan, L. Y. Y., Leung, J. C. K., Lan, H. Y., Lai, K. N. & Tang, S. C. W., Feb 1 2016, In: Kidney International. 89, 2, p. 386-398 13 p.

Research output: Contribution to journal › Article › peer-review

Open Access

88 Scopus citations

2015

BMP7 reduces inflammation and oxidative stress in diabetic tubulopathy

Li, R. X., Yiu, W. H., Wu, H. J., Wong, D. W. L., Chan, L. Y. Y., Lin, M., Leung, J. C. K., Lai, K. N. & Tang, S. C. W., 2015, In: Clinical Science. 128, 4, p. 269-280 12 p.

Research output: Contribution to journal › Article › peer-review

Open Access

36 Scopus citations

N-Acetyl-seryl-aspartyl-lysyl-proline Alleviates Renal Fibrosis Induced by Unilateral Ureteric Obstruction in BALB/C Mice

Chan, G. C. W., Yiu, W. H., Wu, H. J., Wong, D. W. L., Lin, M., Huang, X. R., Lan, H. Y. & Tang, S. C. W., 2015, In: Mediators of Inflammation. 2015, 283123.

Research output: Contribution to journal › Article › peer-review

Open Access

11 Scopus citations

2014

Mesenchymal stem cells modulate albumin-induced renal tubular inflammation and fibrosis

Wu, H. J., Yiu, W. H., Li, R. X., Wong, D. W. L., Leung, J. C. K., Chan, L. Y. Y., Zhang, Y., Lian, Q., Lin, M., Tse, H. F., Lai, K. N. & Tang, S. C. W., Mar 19 2014, In: PloS one. 9, 3, e90883.

Research output: Contribution to journal › Article › peer-review

Open Access

69 Scopus citations

2013

The TLR4 antagonist CRX-526 protects against advanced diabetic nephropathy

Lin, M., Yiu, W. H., Li, R. X., Wu, H. J., Wong, D. W. L., Chan, L. Y. Y., Leung, J. C. K., Lai, K. N. & Tang, S. C. W., May 2013, In: Kidney International. 83, 5, p. 887-900 14 p.

Research output: Contribution to journal › Article › peer-review

Open Access

116 Scopus citations

2012

Toll-like receptor 4 promotes tubular inflammation in diabetic nephropathy

Lin, M., Yiu, W. H., Wu, H. J., Chan, L. Y. Y., Leung, J. C. K., Au, W. S., Chan, K. W., Lai, K. N. & Tang, S. C. W., Jan 2012, In: Journal of the American Society of Nephrology. 23, 1, p. 86-102 17 p.

Research output: Contribution to journal › Article › peer-review

Open Access

344 Scopus citations

2009

Isolation and culture of primary bovine embryonic stem cell colonies by a novel method

Cao, S., Wang, F., Chen, Z., Liu, Z., Mei, C., Wu, H., Huang, J., Li, C., Zhou, L. & Lin, L., Jun 1 2009, In: Journal of Experimental Zoology Part A: Ecological Genetics and Physiology. 311, 5, p. 368-376 9 p.

Research output: Contribution to journal › Article › peer-review

46 Scopus citations

Haojia Wu

Research output

Search results