David Kast

Assistant Professor of Cell Biology and Physiology

    • 533 Citations
    20072019

    Research output per year

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    Personal profile

    Research interests

    The goal of the Kast lab is to understand fundamental cellular and molecular mechanisms that drive the biogenesis and dynamics of intracellular membrane compartments, including endocytic vesicles, the endoplasmic reticulum, the Golgi apparatus, and mitochondria. Cytoskeleton dynamics plays an essential role in the shaping and trafficking of these different membrane structures, however cannot remodel membranes directly. In order to harness the power of the cytoskeleton for the purpose of membrane shaping, cells use diverse sets of modular proteins that form protein complexes with cytoskeleton and signaling proteins, which in turn can partition membranes into autonomous membrane micro-domains. My lab is focused on (1) understanding fundamental molecular mechanisms that govern intracellular membrane reorganization and protein sorting; (2) stimulating the development of fluorescence based assays to delineate important signaling pathways involved in the biogenesis and trafficking vesicle; (3) defining the role(s) of endocytic dysfunction in cancer and neurological disease and take advantage of endocytic pathways to deliver next generation therapeutics to diseased cells. To accomplish these goals, the Kast lab employs a multifaceted approach that interleaves biochemical kinetics, fluorescence spectroscopy, x-ray crystallography, in vitro reconstitution, and live-cell imaging.

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    Research Output

    • 533 Citations
    • 14 Article
    • 2 Review article
    • 1 Chapter

    IRSp53 coordinates AMPK and 14-3-3 signaling to regulate filopodia dynamics and directed cell migration

    Kast, D. J. & Dominguez, R., Jan 1 2019, In : Molecular biology of the cell. 30, 11, p. 1285-1297 13 p.

    Research output: Contribution to journalArticle

    Open Access
  • 3 Scopus citations

    Mechanism of IRSp53 inhibition by 14-3-3

    Kast, D. J. & Dominguez, R., Dec 1 2019, In : Nature communications. 10, 1, 483.

    Research output: Contribution to journalArticle

    Open Access
  • 6 Scopus citations

    The Cytoskeleton–Autophagy Connection

    Kast, D. J. & Dominguez, R., Apr 24 2017, In : Current Biology. 27, 8, p. R318-R326

    Research output: Contribution to journalReview article

    Open Access
  • 64 Scopus citations

    WHAMM Directs the Arp2/3 Complex to the ER for Autophagosome Biogenesis through an Actin Comet Tail Mechanism

    Kast, D. J., Zajac, A. L., Holzbaur, E. L. F., Ostap, E. M. & Dominguez, R., Jun 29 2015, In : Current Biology. 25, 13, p. 1791-1797 7 p.

    Research output: Contribution to journalArticle

    Open Access
  • 51 Scopus citations

    WHAMM links actin assembly via the Arp2/3 complex to autophagy

    Kast, D. J. & Dominguez, R., Jan 1 2015, In : Autophagy. 11, 9, p. 1702-1704 3 p.

    Research output: Contribution to journalArticle

    Open Access
  • 11 Scopus citations