Alex Evers

Henry Elliot Mallinckrodt Professor of Anesthesiology, Head of the Department of Anesthesiology, Professor of Medicine, Professor of Developmental Biology

    • 5167 Citations
    1974 …2020

    Research output per year

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    Personal profile

    Research interests

    My laboratory focuses on the protein chemistry of GABAA receptors to identify and characterize drug binding sites, sequence variants and post-translational modifications as well as to define the protein complexes in which GABAA receptors reside in vivo. A variety of biochemical and molecular biological approaches are applied to these problems, with particular emphasis on high-resolution mass spectrometry. The lab has a longstanding interest in identifying the sites to which anesthetics bind to produce their effects. We have focused on anesthetic neurosteroids, using neurosteroid analogue photolabeling reagents to covalently label protein binding sites and identifying the sites of incorporation using protein mass spectrometry. The lab has also pioneered methods for mass spectrometric methods to sequence the transmembrane segments of native GABAA receptor from picomole quantities of protein. Current directions in the lab include: 1) Studies of dynamic regulation of protein phosphorylation (particularly ion channels) in native brain using global (“shotgun”) mass spectrometric techniques and 2) Identification of GABAA-receptor associated proteins in brain using immunopurification coupled to mass spectrometry with label-free quantitation.

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    Research Output

    Site-specific effects of neurosteroids on gabaa receptor activation and desensitization

    Sugasawa, Y., Cheng, W. W. L., Bracamontes, J. R., Chen, Z. W., Wang, L., Germann, A. L., Pierce, S. R., Senneff, T. C., Krishnan, K., Reichert, D. E., Covey, D. F., Akk, G. & Evers, A. S., Sep 2020, In : eLife. 9, p. 1-32 32 p., e55331.

    Research output: Contribution to journalArticle

    Open Access
  • Common binding sites for cholesterol and neurosteroids on a pentameric ligand-gated ion channel

    Budelier, M. M., Cheng, W. W. L., Chen, Z. W., Bracamontes, J. R., Sugasawa, Y., Krishnan, K., Mydock-McGrane, L., Covey, D. F. & Evers, A. S., Feb 2019, In : Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids. 1864, 2, p. 128-136 9 p.

    Research output: Contribution to journalArticle

  • 6 Scopus citations

    Multiple functional neurosteroid binding sites on gabaa receptors

    Chen, Z. W., Bracamontes, J. R., Budelier, M. M., Germann, A. L., Shin, D. J., Kathiresan, K., Qian, M. X., Manion, B., Cheng, W. W. L., Reichert, D. E., Akk, G., Covey, D. F. & Evers, A. S., Mar 2019, In : PLoS biology. 17, 3, e3000157.

    Research output: Contribution to journalArticle

    Open Access
  • 17 Scopus citations

    Multiple neurosteroid and cholesterol binding sites in voltage-dependent anion channel-1 determined by photo-affinity labeling

    Cheng, W. W. L., Budelier, M. M., Sugasawa, Y., Bergdoll, L., Queralt-Martín, M., Rosencrans, W., Rostovtseva, T. K., Chen, Z. W., Abramson, J., Krishnan, K., Covey, D. F., Whitelegge, J. P. & Evers, A. S., Oct 2019, In : Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids. 1864, 10, p. 1269-1279 11 p.

    Research output: Contribution to journalArticle

  • 7 Scopus citations

    The molecular determinants of neurosteroid binding in the GABA(A) receptor

    Sugasawa, Y., Bracamontes, J. R., Krishnan, K., Covey, D. F., Reichert, D. E., Akk, G., Chen, Q., Tang, P., Evers, A. S. & Cheng, W. W. L., Sep 2019, In : Journal of Steroid Biochemistry and Molecular Biology. 192, 105383.

    Research output: Contribution to journalArticle

  • 2 Scopus citations